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Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation
Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been extensively studied, especially Saccharomyces cerevisiae. However, to date, very little is known about the potential beneficial effects of molds on gut health as part of gut microbiota. We undertook a compre...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504484/ https://www.ncbi.nlm.nih.gov/pubmed/36135618 http://dx.doi.org/10.3390/jof8090893 |
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author | Poirier, Maxime Hugot, Cindy Spatz, Madeleine Da Costa, Gregory Lapiere, Alexia Michaudel, Chloé Danne, Camille Martin, Valérie Langella, Philippe Michel, Marie-Laure Sokol, Harry Boyaval, Patrick Richard, Mathias L. |
author_facet | Poirier, Maxime Hugot, Cindy Spatz, Madeleine Da Costa, Gregory Lapiere, Alexia Michaudel, Chloé Danne, Camille Martin, Valérie Langella, Philippe Michel, Marie-Laure Sokol, Harry Boyaval, Patrick Richard, Mathias L. |
author_sort | Poirier, Maxime |
collection | PubMed |
description | Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been extensively studied, especially Saccharomyces cerevisiae. However, to date, very little is known about the potential beneficial effects of molds on gut health as part of gut microbiota. We undertook a comprehensive characterization of five mold strains, Penicillium camemberti, P. nalgiovense, P. roqueforti, Fusarium domesticum, and Geotrichum candidum used in food processes, on their ability to trigger or protect intestinal inflammation using in vitro human cell models and in vivo susceptibility to sodium dextran sulfate-induced colitis. Comparison of spore adhesion to epithelial cells showed a very wide disparity in results, with F. domesticum and P. roqueforti being the two extremes, with almost no adhesion and 20% adhesion, respectively. Interaction with human immune cells showed mild pro-inflammatory properties of all Penicillium strains and no effect of the others. However, the potential anti-inflammatory abilities detected for G. candidum in vitro were not confirmed in vivo after oral gavage to mice before and during induced colitis. According to the different series of experiments carried out in this study, the impact of the spores of these molds used in food production is limited, with no specific beneficial or harmful effect on the gut. |
format | Online Article Text |
id | pubmed-9504484 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95044842022-09-24 Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation Poirier, Maxime Hugot, Cindy Spatz, Madeleine Da Costa, Gregory Lapiere, Alexia Michaudel, Chloé Danne, Camille Martin, Valérie Langella, Philippe Michel, Marie-Laure Sokol, Harry Boyaval, Patrick Richard, Mathias L. J Fungi (Basel) Article Food processes use different microorganisms, from bacteria to fungi. Yeast strains have been extensively studied, especially Saccharomyces cerevisiae. However, to date, very little is known about the potential beneficial effects of molds on gut health as part of gut microbiota. We undertook a comprehensive characterization of five mold strains, Penicillium camemberti, P. nalgiovense, P. roqueforti, Fusarium domesticum, and Geotrichum candidum used in food processes, on their ability to trigger or protect intestinal inflammation using in vitro human cell models and in vivo susceptibility to sodium dextran sulfate-induced colitis. Comparison of spore adhesion to epithelial cells showed a very wide disparity in results, with F. domesticum and P. roqueforti being the two extremes, with almost no adhesion and 20% adhesion, respectively. Interaction with human immune cells showed mild pro-inflammatory properties of all Penicillium strains and no effect of the others. However, the potential anti-inflammatory abilities detected for G. candidum in vitro were not confirmed in vivo after oral gavage to mice before and during induced colitis. According to the different series of experiments carried out in this study, the impact of the spores of these molds used in food production is limited, with no specific beneficial or harmful effect on the gut. MDPI 2022-08-23 /pmc/articles/PMC9504484/ /pubmed/36135618 http://dx.doi.org/10.3390/jof8090893 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Poirier, Maxime Hugot, Cindy Spatz, Madeleine Da Costa, Gregory Lapiere, Alexia Michaudel, Chloé Danne, Camille Martin, Valérie Langella, Philippe Michel, Marie-Laure Sokol, Harry Boyaval, Patrick Richard, Mathias L. Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation |
title | Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation |
title_full | Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation |
title_fullStr | Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation |
title_full_unstemmed | Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation |
title_short | Effects of Five Filamentous Fungi Used in Food Processes on In Vitro and In Vivo Gut Inflammation |
title_sort | effects of five filamentous fungi used in food processes on in vitro and in vivo gut inflammation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504484/ https://www.ncbi.nlm.nih.gov/pubmed/36135618 http://dx.doi.org/10.3390/jof8090893 |
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