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Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene
The type of soft rice with low amylose content (AC) is more and more favored by consumers for its better eating and cooking quality, as people’s quality of life continuously improves in China. The Wx gene regulates the AC of rice grains, thus affecting the degree of softness of the rice. Mei Meng B...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504814/ https://www.ncbi.nlm.nih.gov/pubmed/36142438 http://dx.doi.org/10.3390/ijms231810517 |
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author | Yang, Jinlian Guo, Xinying Wang, Xuan Fang, Yaoyu Liu, Fang Qin, Baoxiang Li, Rongbai |
author_facet | Yang, Jinlian Guo, Xinying Wang, Xuan Fang, Yaoyu Liu, Fang Qin, Baoxiang Li, Rongbai |
author_sort | Yang, Jinlian |
collection | PubMed |
description | The type of soft rice with low amylose content (AC) is more and more favored by consumers for its better eating and cooking quality, as people’s quality of life continuously improves in China. The Wx gene regulates the AC of rice grains, thus affecting the degree of softness of the rice. Mei Meng B (MMB), Tian Kang B (TKB), and DR462 are three indica rice maintained lines with good morphological characters, but also with undesirably high AC. Therefore, CRISPR/Cas9 technology was used to edit the Wx gene of these lines to create a batch of soft rice breeding materials. New gene-edited lines MMB-10-2, TKB-21-12, and DR462-9-9, derived from the above parental lines, respectively, were selected in the T(2) generations, with an AC of 17.2%, 16.8%, and 17.8%, and gel consistency (GC) of 78.6 mm, 77.4 mm, and 79.6 mm, respectively. The rapid viscosity analysis (RVA) spectrum showed that the three edited lines had a better eating quality as compared to the corresponding wild type, and showing new characteristics, different from the high-quality soft rice popular in the market. There was no significant difference in the main agronomic traits in the three edited lines compared to the corresponding wild types. Moreover, the chalkiness of DR462-9-9 was reduced, resulting in an improved appearance of its polished rice. The present study created soft rice germplasms for breeding improved quality hybrid rice, without changing the excellent traits of their corresponding wild type varieties. |
format | Online Article Text |
id | pubmed-9504814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-95048142022-09-24 Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene Yang, Jinlian Guo, Xinying Wang, Xuan Fang, Yaoyu Liu, Fang Qin, Baoxiang Li, Rongbai Int J Mol Sci Article The type of soft rice with low amylose content (AC) is more and more favored by consumers for its better eating and cooking quality, as people’s quality of life continuously improves in China. The Wx gene regulates the AC of rice grains, thus affecting the degree of softness of the rice. Mei Meng B (MMB), Tian Kang B (TKB), and DR462 are three indica rice maintained lines with good morphological characters, but also with undesirably high AC. Therefore, CRISPR/Cas9 technology was used to edit the Wx gene of these lines to create a batch of soft rice breeding materials. New gene-edited lines MMB-10-2, TKB-21-12, and DR462-9-9, derived from the above parental lines, respectively, were selected in the T(2) generations, with an AC of 17.2%, 16.8%, and 17.8%, and gel consistency (GC) of 78.6 mm, 77.4 mm, and 79.6 mm, respectively. The rapid viscosity analysis (RVA) spectrum showed that the three edited lines had a better eating quality as compared to the corresponding wild type, and showing new characteristics, different from the high-quality soft rice popular in the market. There was no significant difference in the main agronomic traits in the three edited lines compared to the corresponding wild types. Moreover, the chalkiness of DR462-9-9 was reduced, resulting in an improved appearance of its polished rice. The present study created soft rice germplasms for breeding improved quality hybrid rice, without changing the excellent traits of their corresponding wild type varieties. MDPI 2022-09-10 /pmc/articles/PMC9504814/ /pubmed/36142438 http://dx.doi.org/10.3390/ijms231810517 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Jinlian Guo, Xinying Wang, Xuan Fang, Yaoyu Liu, Fang Qin, Baoxiang Li, Rongbai Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene |
title | Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene |
title_full | Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene |
title_fullStr | Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene |
title_full_unstemmed | Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene |
title_short | Development of Soft Rice Lines by Regulating Amylose Content via Editing the 5′UTR of the Wx Gene |
title_sort | development of soft rice lines by regulating amylose content via editing the 5′utr of the wx gene |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9504814/ https://www.ncbi.nlm.nih.gov/pubmed/36142438 http://dx.doi.org/10.3390/ijms231810517 |
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