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Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry

The extracellular accumulation of aggregated amyloid-β (Aβ) in the brain leads to the early pathology of Alzheimer’s disease (AD). The administration of exogenous plant-type ceramides into AD model mice can promote the release of neuronal exosomes, a subtype of extracellular vesicles, that can media...

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Autores principales: Murai, Yuta, Honda, Takumi, Yuyama, Kohei, Mikami, Daisuke, Eguchi, Koichi, Ukawa, Yuichi, Usuki, Seigo, Igarashi, Yasuyuki, Monde, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505575/
https://www.ncbi.nlm.nih.gov/pubmed/36142663
http://dx.doi.org/10.3390/ijms231810751
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author Murai, Yuta
Honda, Takumi
Yuyama, Kohei
Mikami, Daisuke
Eguchi, Koichi
Ukawa, Yuichi
Usuki, Seigo
Igarashi, Yasuyuki
Monde, Kenji
author_facet Murai, Yuta
Honda, Takumi
Yuyama, Kohei
Mikami, Daisuke
Eguchi, Koichi
Ukawa, Yuichi
Usuki, Seigo
Igarashi, Yasuyuki
Monde, Kenji
author_sort Murai, Yuta
collection PubMed
description The extracellular accumulation of aggregated amyloid-β (Aβ) in the brain leads to the early pathology of Alzheimer’s disease (AD). The administration of exogenous plant-type ceramides into AD model mice can promote the release of neuronal exosomes, a subtype of extracellular vesicles, that can mediate Aβ clearance. In vitro studies showed that the length of fatty acids in mammalian-type ceramides is crucial for promoting neuronal exosome release. Therefore, investigating the structures of plant ceramides is important for evaluating the potential in releasing exosomes to remove Aβ. In this study, we assessed plant ceramide species with D-erythro-(4E,8Z)-sphingadienine and D-erythro-(8Z)-phytosphingenine as sphingoid bases that differ from mammalian-type species. Some plant ceramides were more effective than mammalian ceramides at stimulating exosome release. In addition, using deuterium chemistry-based lipidomics, most exogenous plant ceramides were confirmed to be derived from exosomes. These results suggest that the ceramide-dependent upregulation of exosome release may promote the release of exogenous ceramides from cells, and plant ceramides with long-chain fatty acids can effectively release neuronal exosomes and prevent AD pathology.
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spelling pubmed-95055752022-09-24 Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry Murai, Yuta Honda, Takumi Yuyama, Kohei Mikami, Daisuke Eguchi, Koichi Ukawa, Yuichi Usuki, Seigo Igarashi, Yasuyuki Monde, Kenji Int J Mol Sci Article The extracellular accumulation of aggregated amyloid-β (Aβ) in the brain leads to the early pathology of Alzheimer’s disease (AD). The administration of exogenous plant-type ceramides into AD model mice can promote the release of neuronal exosomes, a subtype of extracellular vesicles, that can mediate Aβ clearance. In vitro studies showed that the length of fatty acids in mammalian-type ceramides is crucial for promoting neuronal exosome release. Therefore, investigating the structures of plant ceramides is important for evaluating the potential in releasing exosomes to remove Aβ. In this study, we assessed plant ceramide species with D-erythro-(4E,8Z)-sphingadienine and D-erythro-(8Z)-phytosphingenine as sphingoid bases that differ from mammalian-type species. Some plant ceramides were more effective than mammalian ceramides at stimulating exosome release. In addition, using deuterium chemistry-based lipidomics, most exogenous plant ceramides were confirmed to be derived from exosomes. These results suggest that the ceramide-dependent upregulation of exosome release may promote the release of exogenous ceramides from cells, and plant ceramides with long-chain fatty acids can effectively release neuronal exosomes and prevent AD pathology. MDPI 2022-09-15 /pmc/articles/PMC9505575/ /pubmed/36142663 http://dx.doi.org/10.3390/ijms231810751 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Murai, Yuta
Honda, Takumi
Yuyama, Kohei
Mikami, Daisuke
Eguchi, Koichi
Ukawa, Yuichi
Usuki, Seigo
Igarashi, Yasuyuki
Monde, Kenji
Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
title Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
title_full Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
title_fullStr Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
title_full_unstemmed Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
title_short Evaluation of Plant Ceramide Species-Induced Exosome Release from Neuronal Cells and Exosome Loading Using Deuterium Chemistry
title_sort evaluation of plant ceramide species-induced exosome release from neuronal cells and exosome loading using deuterium chemistry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9505575/
https://www.ncbi.nlm.nih.gov/pubmed/36142663
http://dx.doi.org/10.3390/ijms231810751
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