Successful i-GONAD in Mice at Early Zygote Stage through In Vivo Electroporation Three Min after Intraoviductal Instillation of CRISPR-Ribonucleoprotein

Improved genome editing via oviductal nucleic acids delivery (i-GONAD) is a new technology enabling in situ genome editing of mammalian zygotes exiting the oviductal lumen, which is now available in mice, rats, and hamsters. In this method, CRISPR/Cas9 genome-editing reagents are delivered directly...

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Detalles Bibliográficos
Autores principales: Takabayashi, Shuji, Iijima, Kenta, Tsujimura, Masumi, Aoshima, Takuya, Takagi, Hisayoshi, Aoto, Kazushi, Sato, Masahiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506528/
https://www.ncbi.nlm.nih.gov/pubmed/36142589
http://dx.doi.org/10.3390/ijms231810678

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9506528/
https://www.ncbi.nlm.nih.gov/pubmed/36142589
http://dx.doi.org/10.3390/ijms231810678

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