The evolutionary advantage of an aromatic clamp in plant family 3 glycoside exo-hydrolases

In the barley β-d-glucan glucohydrolase, a glycoside hydrolase family 3 (GH3) enzyme, the Trp286/Trp434 clamp ensures β-d-glucosides binding, which is fundamental for substrate hydrolysis during plant growth and development. We employ mutagenesis, high-resolution X-ray crystallography, and multi-sca...

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Detalles Bibliográficos
Autores principales: Luang, Sukanya, Fernández-Luengo, Xavier, Nin-Hill, Alba, Streltsov, Victor A., Schwerdt, Julian G., Alonso-Gil, Santiago, Ketudat Cairns, James R., Pradeau, Stéphanie, Fort, Sébastien, Maréchal, Jean-Didier, Masgrau, Laura, Rovira, Carme, Hrmova, Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508125/
https://www.ncbi.nlm.nih.gov/pubmed/36151080
http://dx.doi.org/10.1038/s41467-022-33180-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508125/
https://www.ncbi.nlm.nih.gov/pubmed/36151080
http://dx.doi.org/10.1038/s41467-022-33180-5

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