Protocol to detect in vitro and in cell ubiquitylation of mitochondrial DNA polymerase gamma by mitochondrial E3 ligase MITOL

Mitochondrial polymerase gamma (PolγA) is the only replicative polymerase in mitochondria. To determine PolγA ubiquitylation in cells, Flag-PolγA and MITOL are overexpressed, and subsequently the immunoprecipitated Flag-PolγA is checked for ubiquitylation. Alternately, in vitro synthesized PolγA and...

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Detalles Bibliográficos
Autores principales: Hussain, Mansoor, Saifi, Shabnam, Mohammed, Aftab, Sengupta, Sagar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508469/
https://www.ncbi.nlm.nih.gov/pubmed/36136752
http://dx.doi.org/10.1016/j.xpro.2022.101710

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508469/
https://www.ncbi.nlm.nih.gov/pubmed/36136752
http://dx.doi.org/10.1016/j.xpro.2022.101710

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