A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles

Here, we present a general protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles (GUVs). In this protocol, we use GUVs to mimic Ago1 protein’s phase separation behavior on the membrane through binding with phosphoinositides (PIPs). We provide procedu...

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Detalles Bibliográficos
Autores principales: Zhu, Yuanxiang, Gao, Yajie, Sun, Qinmiao, Chen, Dahua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508480/
https://www.ncbi.nlm.nih.gov/pubmed/36136754
http://dx.doi.org/10.1016/j.xpro.2022.101709

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508480/
https://www.ncbi.nlm.nih.gov/pubmed/36136754
http://dx.doi.org/10.1016/j.xpro.2022.101709

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