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A protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles
Here, we present a general protocol for mimicking lipid-mediated phase separation on the membrane using giant unilamellar vesicles (GUVs). In this protocol, we use GUVs to mimic Ago1 protein’s phase separation behavior on the membrane through binding with phosphoinositides (PIPs). We provide procedu...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508480/ https://www.ncbi.nlm.nih.gov/pubmed/36136754 http://dx.doi.org/10.1016/j.xpro.2022.101709 |