Protocol to measure cleavage efficiency of the meiotic cohesin subunit Rec8 by separase in mouse oocytes using a biosensor

Here, we describe a biosensor to assess meiotic cohesin subunit Rec8 cleavage in mouse oocytes. We detail oocyte collection and microinjection of the mRNA expressing the biosensor. The biosensor is targeted to chromosomes and consists of two fluorophores flanking a Rec8 fragment containing separase...

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Detalles Bibliográficos
Autores principales: Nikalayevich, Elvira, Wassmann, Katja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508607/
https://www.ncbi.nlm.nih.gov/pubmed/36149797
http://dx.doi.org/10.1016/j.xpro.2022.101714

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508607/
https://www.ncbi.nlm.nih.gov/pubmed/36149797
http://dx.doi.org/10.1016/j.xpro.2022.101714

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