Protocol for live-cell fluorescence-guided cryoFIB-milling and electron cryo-tomography of virus-infected cells

Here, we present a protocol for assessing virus-infected cells using electron cryo-tomography (cryoET). It includes the basic workflows of seeding cells, plunge-freezing, clipping, cryo-focused ion beam milling (cryoFIB-milling), and cryoET, as well as two optional modules: micropatterning and live-...

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Detalles Bibliográficos
Autores principales: Franken, Linda E., Rosch, Rene, Laugks, Ulrike, Grünewald, Kay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508610/
https://www.ncbi.nlm.nih.gov/pubmed/36149798
http://dx.doi.org/10.1016/j.xpro.2022.101696

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508610/
https://www.ncbi.nlm.nih.gov/pubmed/36149798
http://dx.doi.org/10.1016/j.xpro.2022.101696

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