Selective ablation of 3′ RNA ends and processive RTs facilitate direct cDNA sequencing of full-length host cell and viral transcripts

Alternative splicing (AS) is necessary for viral proliferation in host cells and a critical regulatory component of viral gene expression. Conventional RNA-seq approaches provide incomplete coverage of AS due to their short read lengths and are susceptible to biases and artifacts introduced in preva...

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Detalles Bibliográficos
Autores principales: Gallardo, Christian M, Nguyen, Anh-Viet T, Routh, Andrew L, Torbett, Bruce E
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508845/
https://www.ncbi.nlm.nih.gov/pubmed/35736235
http://dx.doi.org/10.1093/nar/gkac516

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508845/
https://www.ncbi.nlm.nih.gov/pubmed/35736235
http://dx.doi.org/10.1093/nar/gkac516

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