Allosteric activation of CRISPR-Cas12a requires the concerted movement of the bridge helix and helix 1 of the RuvC II domain

Nucleases derived from the prokaryotic defense system CRISPR-Cas are frequently re-purposed for gene editing and molecular diagnostics. Hence, an in-depth understanding of the molecular mechanisms of these enzymes is of crucial importance. We focused on Cas12a from Francisella novicida (FnCas12a) an...

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Detalles Bibliográficos
Autores principales: Wörle, Elisabeth, Newman, Anthony, D’Silva, Jovita, Burgio, Gaetan, Grohmann, Dina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508855/
https://www.ncbi.nlm.nih.gov/pubmed/36107767
http://dx.doi.org/10.1093/nar/gkac767

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9508855/
https://www.ncbi.nlm.nih.gov/pubmed/36107767
http://dx.doi.org/10.1093/nar/gkac767

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