Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay

Monitoring antigen-specific T cell frequency, function, and phenotype is essential to assess the host immune response to pathogens or novel vaccines. Here, we describe a rapid and simple ex vivo whole blood assay to detect and phenotype the SARS-CoV-2-specific T cell response. We detail steps for wh...

Descripción completa

Detalles Bibliográficos
Autores principales: Mutavhatsindi, Hygon, Riou, Catherine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510067/
https://www.ncbi.nlm.nih.gov/pubmed/36272131
http://dx.doi.org/10.1016/j.xpro.2022.101771
_version_ 1784797370487144448
author Mutavhatsindi, Hygon
Riou, Catherine
author_facet Mutavhatsindi, Hygon
Riou, Catherine
author_sort Mutavhatsindi, Hygon
collection PubMed
description Monitoring antigen-specific T cell frequency, function, and phenotype is essential to assess the host immune response to pathogens or novel vaccines. Here, we describe a rapid and simple ex vivo whole blood assay to detect and phenotype the SARS-CoV-2-specific T cell response. We detail steps for whole blood stimulation with SARS-CoV-2 spike peptide and subsequent cell fixation and cryopreservation. We further describe thawing and cell staining steps for flow cytometry analysis. This approach minimizes sample manipulation and has a quick turnaround time. For complete details on the use and execution of this protocol, please refer to Riou et al. (2021).
format Online
Article
Text
id pubmed-9510067
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-95100672022-09-26 Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay Mutavhatsindi, Hygon Riou, Catherine STAR Protoc Protocol Monitoring antigen-specific T cell frequency, function, and phenotype is essential to assess the host immune response to pathogens or novel vaccines. Here, we describe a rapid and simple ex vivo whole blood assay to detect and phenotype the SARS-CoV-2-specific T cell response. We detail steps for whole blood stimulation with SARS-CoV-2 spike peptide and subsequent cell fixation and cryopreservation. We further describe thawing and cell staining steps for flow cytometry analysis. This approach minimizes sample manipulation and has a quick turnaround time. For complete details on the use and execution of this protocol, please refer to Riou et al. (2021). Elsevier 2022-09-26 /pmc/articles/PMC9510067/ /pubmed/36272131 http://dx.doi.org/10.1016/j.xpro.2022.101771 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Mutavhatsindi, Hygon
Riou, Catherine
Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay
title Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay
title_full Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay
title_fullStr Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay
title_full_unstemmed Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay
title_short Protocol to quantify and phenotype SARS-CoV-2-specific T cell response using a rapid flow-cytometry-based whole blood assay
title_sort protocol to quantify and phenotype sars-cov-2-specific t cell response using a rapid flow-cytometry-based whole blood assay
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510067/
https://www.ncbi.nlm.nih.gov/pubmed/36272131
http://dx.doi.org/10.1016/j.xpro.2022.101771
work_keys_str_mv AT mutavhatsindihygon protocoltoquantifyandphenotypesarscov2specifictcellresponseusingarapidflowcytometrybasedwholebloodassay
AT rioucatherine protocoltoquantifyandphenotypesarscov2specifictcellresponseusingarapidflowcytometrybasedwholebloodassay

Ejemplares similares