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MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation

Rett syndrome is a human intellectual disability disorder that is associated with mutations in the X-linked MECP2 gene. The epigenetic reader MeCP2 binds to methylated cytosines on the DNA and regulates chromatin organization. We have shown previously that MECP2 Rett syndrome missense mutations are...

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Autores principales: Schmidt, Annika, Frei, Jana, Poetsch, Ansgar, Chittka, Alexandra, Zhang, Hui, Aßmann, Chris, Lehmkuhl, Anne, Bauer, Uta-Maria, Nuber, Ulrike A., Cardoso, M. Cristina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510713/
https://www.ncbi.nlm.nih.gov/pubmed/36172281
http://dx.doi.org/10.3389/fcell.2022.941493
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author Schmidt, Annika
Frei, Jana
Poetsch, Ansgar
Chittka, Alexandra
Zhang, Hui
Aßmann, Chris
Lehmkuhl, Anne
Bauer, Uta-Maria
Nuber, Ulrike A.
Cardoso, M. Cristina
author_facet Schmidt, Annika
Frei, Jana
Poetsch, Ansgar
Chittka, Alexandra
Zhang, Hui
Aßmann, Chris
Lehmkuhl, Anne
Bauer, Uta-Maria
Nuber, Ulrike A.
Cardoso, M. Cristina
author_sort Schmidt, Annika
collection PubMed
description Rett syndrome is a human intellectual disability disorder that is associated with mutations in the X-linked MECP2 gene. The epigenetic reader MeCP2 binds to methylated cytosines on the DNA and regulates chromatin organization. We have shown previously that MECP2 Rett syndrome missense mutations are impaired in chromatin binding and heterochromatin reorganization. Here, we performed a proteomics analysis of post-translational modifications of MeCP2 isolated from adult mouse brain. We show that MeCP2 carries various post-translational modifications, among them phosphorylation on S80 and S421, which lead to minor changes in either heterochromatin binding kinetics or clustering. We found that MeCP2 is (di)methylated on several arginines and that this modification alters heterochromatin organization. Interestingly, we identified the Rett syndrome mutation site R106 as a dimethylation site. In addition, co-expression of protein arginine methyltransferases (PRMT)1 and PRMT6 lead to a decrease of heterochromatin clustering. Altogether, we identified and validated novel modifications of MeCP2 in the brain and show that these can modulate its ability to bind as well as reorganize heterochromatin, which may play a role in the pathology of Rett syndrome.
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spelling pubmed-95107132022-09-27 MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation Schmidt, Annika Frei, Jana Poetsch, Ansgar Chittka, Alexandra Zhang, Hui Aßmann, Chris Lehmkuhl, Anne Bauer, Uta-Maria Nuber, Ulrike A. Cardoso, M. Cristina Front Cell Dev Biol Cell and Developmental Biology Rett syndrome is a human intellectual disability disorder that is associated with mutations in the X-linked MECP2 gene. The epigenetic reader MeCP2 binds to methylated cytosines on the DNA and regulates chromatin organization. We have shown previously that MECP2 Rett syndrome missense mutations are impaired in chromatin binding and heterochromatin reorganization. Here, we performed a proteomics analysis of post-translational modifications of MeCP2 isolated from adult mouse brain. We show that MeCP2 carries various post-translational modifications, among them phosphorylation on S80 and S421, which lead to minor changes in either heterochromatin binding kinetics or clustering. We found that MeCP2 is (di)methylated on several arginines and that this modification alters heterochromatin organization. Interestingly, we identified the Rett syndrome mutation site R106 as a dimethylation site. In addition, co-expression of protein arginine methyltransferases (PRMT)1 and PRMT6 lead to a decrease of heterochromatin clustering. Altogether, we identified and validated novel modifications of MeCP2 in the brain and show that these can modulate its ability to bind as well as reorganize heterochromatin, which may play a role in the pathology of Rett syndrome. Frontiers Media S.A. 2022-09-12 /pmc/articles/PMC9510713/ /pubmed/36172281 http://dx.doi.org/10.3389/fcell.2022.941493 Text en Copyright © 2022 Schmidt, Frei, Poetsch, Chittka, Zhang, Aßmann, Lehmkuhl, Bauer, Nuber and Cardoso. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Schmidt, Annika
Frei, Jana
Poetsch, Ansgar
Chittka, Alexandra
Zhang, Hui
Aßmann, Chris
Lehmkuhl, Anne
Bauer, Uta-Maria
Nuber, Ulrike A.
Cardoso, M. Cristina
MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
title MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
title_full MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
title_fullStr MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
title_full_unstemmed MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
title_short MeCP2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
title_sort mecp2 heterochromatin organization is modulated by arginine methylation and serine phosphorylation
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9510713/
https://www.ncbi.nlm.nih.gov/pubmed/36172281
http://dx.doi.org/10.3389/fcell.2022.941493
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