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Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer
Endometrial cancer is one of the most frequently diagnosed gynecological cancers worldwide, and its prevalence has increased by more than 50% over the last two decades. Despite the understanding of the major signaling pathways driving the growth and metastasis of endometrial cancer, clinical trials...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512672/ https://www.ncbi.nlm.nih.gov/pubmed/36103879 http://dx.doi.org/10.1016/j.xcrm.2022.100738 |
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author | Jamaluddin, M. Fairuz B. Ko, Yi-An Ghosh, Arnab Syed, Shafiq M. Ius, Yvette O’Sullivan, Rachel Netherton, Jacob K. Baker, Mark A. Nahar, Pravin Jaaback, Kenneth Tanwar, Pradeep S. |
author_facet | Jamaluddin, M. Fairuz B. Ko, Yi-An Ghosh, Arnab Syed, Shafiq M. Ius, Yvette O’Sullivan, Rachel Netherton, Jacob K. Baker, Mark A. Nahar, Pravin Jaaback, Kenneth Tanwar, Pradeep S. |
author_sort | Jamaluddin, M. Fairuz B. |
collection | PubMed |
description | Endometrial cancer is one of the most frequently diagnosed gynecological cancers worldwide, and its prevalence has increased by more than 50% over the last two decades. Despite the understanding of the major signaling pathways driving the growth and metastasis of endometrial cancer, clinical trials targeting these signals have reported poor outcomes. The heterogeneous nature of endometrial cancer is suspected to be one of the key reasons for the failure of targeted therapies. In this study, we perform a sequential window acquisition of all theoretical fragment ion spectra (SWATH)-based comparative proteomic analysis of 63 tumor biopsies collected from 20 patients and define differences in protein signature in multiple regions of the same tumor. We develop organoids from multiple biopsies collected from the same tumor and show that organoids capture heterogeneity in endometrial cancer growth. Overall, using quantitative proteomics and patient-derived organoids, we define the heterogeneous nature of endometrial cancer within a patient’s tumor. |
format | Online Article Text |
id | pubmed-9512672 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-95126722022-09-28 Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer Jamaluddin, M. Fairuz B. Ko, Yi-An Ghosh, Arnab Syed, Shafiq M. Ius, Yvette O’Sullivan, Rachel Netherton, Jacob K. Baker, Mark A. Nahar, Pravin Jaaback, Kenneth Tanwar, Pradeep S. Cell Rep Med Article Endometrial cancer is one of the most frequently diagnosed gynecological cancers worldwide, and its prevalence has increased by more than 50% over the last two decades. Despite the understanding of the major signaling pathways driving the growth and metastasis of endometrial cancer, clinical trials targeting these signals have reported poor outcomes. The heterogeneous nature of endometrial cancer is suspected to be one of the key reasons for the failure of targeted therapies. In this study, we perform a sequential window acquisition of all theoretical fragment ion spectra (SWATH)-based comparative proteomic analysis of 63 tumor biopsies collected from 20 patients and define differences in protein signature in multiple regions of the same tumor. We develop organoids from multiple biopsies collected from the same tumor and show that organoids capture heterogeneity in endometrial cancer growth. Overall, using quantitative proteomics and patient-derived organoids, we define the heterogeneous nature of endometrial cancer within a patient’s tumor. Elsevier 2022-09-13 /pmc/articles/PMC9512672/ /pubmed/36103879 http://dx.doi.org/10.1016/j.xcrm.2022.100738 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Jamaluddin, M. Fairuz B. Ko, Yi-An Ghosh, Arnab Syed, Shafiq M. Ius, Yvette O’Sullivan, Rachel Netherton, Jacob K. Baker, Mark A. Nahar, Pravin Jaaback, Kenneth Tanwar, Pradeep S. Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
title | Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
title_full | Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
title_fullStr | Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
title_full_unstemmed | Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
title_short | Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
title_sort | proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512672/ https://www.ncbi.nlm.nih.gov/pubmed/36103879 http://dx.doi.org/10.1016/j.xcrm.2022.100738 |
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