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High-yield production in Escherichia coli and convenient purification of a candidate vaccine against SARS-CoV-2
OBJECTIVES: The aim of the present work was to identify a time-saving, effective, and low-cost strategy to produce in Escherichia coli a protein chimera representing a fusion anti-SARS-CoV-2 candidate vaccine, consisting of immunogenic and antigenic moieties. RESULTS: We overexpressed in E. coli BL2...
Autores principales: | Maltoni, Giulia, Scutteri, Lorenzo, Mensitieri, Francesca, Piaz, Fabrizio Dal, Hochkoeppler, Alejandro |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9512991/ https://www.ncbi.nlm.nih.gov/pubmed/36161539 http://dx.doi.org/10.1007/s10529-022-03298-z |
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