One-Step Genotyping Method in loxP-Based Conditional Knockout Mice Generated by CRISPR-Cas9 Technology

With the development of CRISPR-Cas9 gene editing and in vitro fertilization (IVF) technology, we can now easily construct genetically modified mouse strains with indels, especially for loxP-based strategy. However, the general genotyping methods are time-consuming and unreliable given the loxP site...

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Detalles Bibliográficos
Autores principales: Zhu, He, Liu, Siqian, He, Wenxi, Sun, Fei, Li, Yang, Yang, Ping, Yu, Qilin, Zhang, Shu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515137/
https://www.ncbi.nlm.nih.gov/pubmed/35503156
http://dx.doi.org/10.1007/s12033-022-00500-5

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9515137/
https://www.ncbi.nlm.nih.gov/pubmed/35503156
http://dx.doi.org/10.1007/s12033-022-00500-5

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