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Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway
OBJECTIVE: To investigate the role and mechanism of protein kinase N2 (PKN2) in hydrogen peroxide (H(2)O(2))-induced injury of PC12 cells. METHOD: s. PC12 cells were transfected with lentivirus to knock down or overexpress PKN2 and then were treated with 300 μM H(2)O(2) to establish a cell model of...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519335/ https://www.ncbi.nlm.nih.gov/pubmed/36185087 http://dx.doi.org/10.1155/2022/2483669 |
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author | Wang, Lin Zhang, Lin |
author_facet | Wang, Lin Zhang, Lin |
author_sort | Wang, Lin |
collection | PubMed |
description | OBJECTIVE: To investigate the role and mechanism of protein kinase N2 (PKN2) in hydrogen peroxide (H(2)O(2))-induced injury of PC12 cells. METHOD: s. PC12 cells were transfected with lentivirus to knock down or overexpress PKN2 and then were treated with 300 μM H(2)O(2) to establish a cell model of oxidative stress injury. The cell viability of PC12 cells in each group was determined by the CCK-8 method. Biochemical assays were used to measure reactive oxygen species (ROS), malondialdehyde (MDA) levels, and superoxide dismutase (SOD) activity. Western blot was used to detect the protein expressions of PKN2, caspase-3, cleaved-caspase-3, PARP, cleaved-PARP, p-mTOR, and mTOR in PC12 cells in each group. RESULTS: H(2)O(2) treatment could significantly reduce PC12 cell viability and promote cell apoptosis and oxidative stress. PKN2 overexpression inhibited H(2)O(2)-induced apoptosis and oxidation damage by increasing PC12 cell viability, SOD activity, and p-mTOR protein expression, reducing intracellular ROS and MDA levels, and cleaved-caspase-3 and cleaved-PARP protein expression. CONCLUSION: PKN2 overexpression can alleviate H(2)O(2)-induced oxidative stress injury and apoptosis in PC12 cells by activating the mTOR pathway. |
format | Online Article Text |
id | pubmed-9519335 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95193352022-09-29 Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway Wang, Lin Zhang, Lin Evid Based Complement Alternat Med Research Article OBJECTIVE: To investigate the role and mechanism of protein kinase N2 (PKN2) in hydrogen peroxide (H(2)O(2))-induced injury of PC12 cells. METHOD: s. PC12 cells were transfected with lentivirus to knock down or overexpress PKN2 and then were treated with 300 μM H(2)O(2) to establish a cell model of oxidative stress injury. The cell viability of PC12 cells in each group was determined by the CCK-8 method. Biochemical assays were used to measure reactive oxygen species (ROS), malondialdehyde (MDA) levels, and superoxide dismutase (SOD) activity. Western blot was used to detect the protein expressions of PKN2, caspase-3, cleaved-caspase-3, PARP, cleaved-PARP, p-mTOR, and mTOR in PC12 cells in each group. RESULTS: H(2)O(2) treatment could significantly reduce PC12 cell viability and promote cell apoptosis and oxidative stress. PKN2 overexpression inhibited H(2)O(2)-induced apoptosis and oxidation damage by increasing PC12 cell viability, SOD activity, and p-mTOR protein expression, reducing intracellular ROS and MDA levels, and cleaved-caspase-3 and cleaved-PARP protein expression. CONCLUSION: PKN2 overexpression can alleviate H(2)O(2)-induced oxidative stress injury and apoptosis in PC12 cells by activating the mTOR pathway. Hindawi 2022-09-21 /pmc/articles/PMC9519335/ /pubmed/36185087 http://dx.doi.org/10.1155/2022/2483669 Text en Copyright © 2022 Lin Wang and Lin Zhang. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Lin Zhang, Lin Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway |
title | Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway |
title_full | Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway |
title_fullStr | Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway |
title_full_unstemmed | Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway |
title_short | Protein Kinase N2 Reduces Hydrogen Peroxide-inducedDamage and Apoptosis in PC12 Cells by AntiOxidative Stress and Activation of the mTOR Pathway |
title_sort | protein kinase n2 reduces hydrogen peroxide-induceddamage and apoptosis in pc12 cells by antioxidative stress and activation of the mtor pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519335/ https://www.ncbi.nlm.nih.gov/pubmed/36185087 http://dx.doi.org/10.1155/2022/2483669 |
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