Design and use of a back splicing junction probe for pulldown of circular RNA-binding proteins in cell lines

Due to the unique structure of circular RNAs, it is challenging to use traditional pulldown approaches. Here, we describe the design and use of a probe that spans the back splicing junction (BSJ), enabling interaction with circular RNAs. The probe repeats four times, allowing efficient and specific...

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Detalles Bibliográficos
Autores principales: Li, Feiya, Wu, Nan, Yang, Qiwei, Du, William W., Qadir, Javeria, Yang, Burton B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519607/
https://www.ncbi.nlm.nih.gov/pubmed/36149796
http://dx.doi.org/10.1016/j.xpro.2022.101702

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519607/
https://www.ncbi.nlm.nih.gov/pubmed/36149796
http://dx.doi.org/10.1016/j.xpro.2022.101702

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