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Design and use of a back splicing junction probe for pulldown of circular RNA-binding proteins in cell lines
Due to the unique structure of circular RNAs, it is challenging to use traditional pulldown approaches. Here, we describe the design and use of a probe that spans the back splicing junction (BSJ), enabling interaction with circular RNAs. The probe repeats four times, allowing efficient and specific...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519607/ https://www.ncbi.nlm.nih.gov/pubmed/36149796 http://dx.doi.org/10.1016/j.xpro.2022.101702 |