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SSNIP-seq: A simple and rapid method for isolation of single-sperm nucleic acid for high-throughput sequencing

We developed a simple and reliable method for the isolation of haploid nuclei from fresh and frozen testes. The described protocol uses readily available reagents in combination with flow cytometry to separate haploid and diploid nuclei. The protocol can be completed within 1 hour and the resulting...

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Detalles Bibliográficos
Autores principales: Novakovic, Stevan, Tsui, Vanessa, Semple, Tim, Martelotto, Luciano, McCarthy, Davis J., Crismani, Wayne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521801/
https://www.ncbi.nlm.nih.gov/pubmed/36173986
http://dx.doi.org/10.1371/journal.pone.0275168