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SSNIP-seq: A simple and rapid method for isolation of single-sperm nucleic acid for high-throughput sequencing
We developed a simple and reliable method for the isolation of haploid nuclei from fresh and frozen testes. The described protocol uses readily available reagents in combination with flow cytometry to separate haploid and diploid nuclei. The protocol can be completed within 1 hour and the resulting...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9521801/ https://www.ncbi.nlm.nih.gov/pubmed/36173986 http://dx.doi.org/10.1371/journal.pone.0275168 |