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Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats

Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarob...

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Autores principales: Shirai, Ikuya, Karasawa, Koji, Kodaira, Yusuke, Iwasaki, Yu, Shigemura, Yasutaka, Makabe, Hidefumi, Katayama, Shigeru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525106/
https://www.ncbi.nlm.nih.gov/pubmed/36185657
http://dx.doi.org/10.3389/fnut.2022.996607
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author Shirai, Ikuya
Karasawa, Koji
Kodaira, Yusuke
Iwasaki, Yu
Shigemura, Yasutaka
Makabe, Hidefumi
Katayama, Shigeru
author_facet Shirai, Ikuya
Karasawa, Koji
Kodaira, Yusuke
Iwasaki, Yu
Shigemura, Yasutaka
Makabe, Hidefumi
Katayama, Shigeru
author_sort Shirai, Ikuya
collection PubMed
description Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarobiose (Abi), agarotetraose (Ate), and agarohexaose (Ahe) were detected in rat plasma after oral administration of AOSs. The detection level of agarobiose in the plasma was higher than that of agarohexaose, which was consistent with the permeation study using Caco-2 cell monolayers. Further, the adenosine triphosphate inhibitor (sodium azide) or endocytosis inhibitor (colchicine) did not inhibit AOS permeation through Caco-2 cell monolayers. Conversely, AOS permeation enhanced upon treatment with cytochalasin B, a tight junction disrupter, suggesting that AOSs might have passed mainly through the tight junctions between the intestinal epithelial cells. These results indicate that AOSs, especially agarobiose, can be absorbed as an intact form via the gastrointestinal tract across the intestinal epithelium through the paracellular pathway.
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spelling pubmed-95251062022-10-01 Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats Shirai, Ikuya Karasawa, Koji Kodaira, Yusuke Iwasaki, Yu Shigemura, Yasutaka Makabe, Hidefumi Katayama, Shigeru Front Nutr Nutrition Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarobiose (Abi), agarotetraose (Ate), and agarohexaose (Ahe) were detected in rat plasma after oral administration of AOSs. The detection level of agarobiose in the plasma was higher than that of agarohexaose, which was consistent with the permeation study using Caco-2 cell monolayers. Further, the adenosine triphosphate inhibitor (sodium azide) or endocytosis inhibitor (colchicine) did not inhibit AOS permeation through Caco-2 cell monolayers. Conversely, AOS permeation enhanced upon treatment with cytochalasin B, a tight junction disrupter, suggesting that AOSs might have passed mainly through the tight junctions between the intestinal epithelial cells. These results indicate that AOSs, especially agarobiose, can be absorbed as an intact form via the gastrointestinal tract across the intestinal epithelium through the paracellular pathway. Frontiers Media S.A. 2022-09-16 /pmc/articles/PMC9525106/ /pubmed/36185657 http://dx.doi.org/10.3389/fnut.2022.996607 Text en Copyright © 2022 Shirai, Karasawa, Kodaira, Iwasaki, Shigemura, Makabe and Katayama. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Shirai, Ikuya
Karasawa, Koji
Kodaira, Yusuke
Iwasaki, Yu
Shigemura, Yasutaka
Makabe, Hidefumi
Katayama, Shigeru
Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
title Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
title_full Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
title_fullStr Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
title_full_unstemmed Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
title_short Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
title_sort intestinal permeability of agaro-oligosaccharides: transport across caco-2 cell monolayers and pharmacokinetics in rats
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525106/
https://www.ncbi.nlm.nih.gov/pubmed/36185657
http://dx.doi.org/10.3389/fnut.2022.996607
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