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Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats
Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarob...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525106/ https://www.ncbi.nlm.nih.gov/pubmed/36185657 http://dx.doi.org/10.3389/fnut.2022.996607 |
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author | Shirai, Ikuya Karasawa, Koji Kodaira, Yusuke Iwasaki, Yu Shigemura, Yasutaka Makabe, Hidefumi Katayama, Shigeru |
author_facet | Shirai, Ikuya Karasawa, Koji Kodaira, Yusuke Iwasaki, Yu Shigemura, Yasutaka Makabe, Hidefumi Katayama, Shigeru |
author_sort | Shirai, Ikuya |
collection | PubMed |
description | Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarobiose (Abi), agarotetraose (Ate), and agarohexaose (Ahe) were detected in rat plasma after oral administration of AOSs. The detection level of agarobiose in the plasma was higher than that of agarohexaose, which was consistent with the permeation study using Caco-2 cell monolayers. Further, the adenosine triphosphate inhibitor (sodium azide) or endocytosis inhibitor (colchicine) did not inhibit AOS permeation through Caco-2 cell monolayers. Conversely, AOS permeation enhanced upon treatment with cytochalasin B, a tight junction disrupter, suggesting that AOSs might have passed mainly through the tight junctions between the intestinal epithelial cells. These results indicate that AOSs, especially agarobiose, can be absorbed as an intact form via the gastrointestinal tract across the intestinal epithelium through the paracellular pathway. |
format | Online Article Text |
id | pubmed-9525106 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95251062022-10-01 Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats Shirai, Ikuya Karasawa, Koji Kodaira, Yusuke Iwasaki, Yu Shigemura, Yasutaka Makabe, Hidefumi Katayama, Shigeru Front Nutr Nutrition Agaro-oligosaccharides (AOSs), even-numbered oligosaccharides prepared from agar, are applied to various food, including supplements, drinks, and jellies because of their biological activities. This study aimed to evaluate the AOS permeation in the gastrointestinal tract in vivo and in vitro. Agarobiose (Abi), agarotetraose (Ate), and agarohexaose (Ahe) were detected in rat plasma after oral administration of AOSs. The detection level of agarobiose in the plasma was higher than that of agarohexaose, which was consistent with the permeation study using Caco-2 cell monolayers. Further, the adenosine triphosphate inhibitor (sodium azide) or endocytosis inhibitor (colchicine) did not inhibit AOS permeation through Caco-2 cell monolayers. Conversely, AOS permeation enhanced upon treatment with cytochalasin B, a tight junction disrupter, suggesting that AOSs might have passed mainly through the tight junctions between the intestinal epithelial cells. These results indicate that AOSs, especially agarobiose, can be absorbed as an intact form via the gastrointestinal tract across the intestinal epithelium through the paracellular pathway. Frontiers Media S.A. 2022-09-16 /pmc/articles/PMC9525106/ /pubmed/36185657 http://dx.doi.org/10.3389/fnut.2022.996607 Text en Copyright © 2022 Shirai, Karasawa, Kodaira, Iwasaki, Shigemura, Makabe and Katayama. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Shirai, Ikuya Karasawa, Koji Kodaira, Yusuke Iwasaki, Yu Shigemura, Yasutaka Makabe, Hidefumi Katayama, Shigeru Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
title | Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
title_full | Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
title_fullStr | Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
title_full_unstemmed | Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
title_short | Intestinal permeability of agaro-oligosaccharides: Transport across Caco-2 cell monolayers and pharmacokinetics in rats |
title_sort | intestinal permeability of agaro-oligosaccharides: transport across caco-2 cell monolayers and pharmacokinetics in rats |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525106/ https://www.ncbi.nlm.nih.gov/pubmed/36185657 http://dx.doi.org/10.3389/fnut.2022.996607 |
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