Protocol to use RNaseH1-based CRISPR to modulate locus-associated R-loops

Modulating R-loop triplex nucleic acid structures reveals their roles across the genome. However, common approaches cannot ascribe functions to R-loops in a locus-associated manner. This protocol presents the use of a locus-associated R-loop-modulating system (dubbed LasR), which employs an inducibl...

Descripción completa

Detalles Bibliográficos
Autores principales: Khosraviani, Negin, Abraham, Karan J., Chan, Janet N.Y., Mekhail, Karim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525952/
https://www.ncbi.nlm.nih.gov/pubmed/36178790
http://dx.doi.org/10.1016/j.xpro.2022.101734

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525952/
https://www.ncbi.nlm.nih.gov/pubmed/36178790
http://dx.doi.org/10.1016/j.xpro.2022.101734

Ejemplares similares