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Protocol to use RNaseH1-based CRISPR to modulate locus-associated R-loops
Modulating R-loop triplex nucleic acid structures reveals their roles across the genome. However, common approaches cannot ascribe functions to R-loops in a locus-associated manner. This protocol presents the use of a locus-associated R-loop-modulating system (dubbed LasR), which employs an inducibl...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9525952/ https://www.ncbi.nlm.nih.gov/pubmed/36178790 http://dx.doi.org/10.1016/j.xpro.2022.101734 |