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Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
Recent studies have shown that CD4(+)CD8αα(+) T cells are induced in the hypoxic environment of the small intestinal epithelium. Herein, we describe a protocol for CD4(+)CD8αα(+) T cell induction from freshly isolated naive CD4(+) T cells, including procedures for the isolation and enrichment of mou...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526228/ https://www.ncbi.nlm.nih.gov/pubmed/36170110 http://dx.doi.org/10.1016/j.xpro.2022.101728 |