Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction

Recent studies have shown that CD4(+)CD8αα(+) T cells are induced in the hypoxic environment of the small intestinal epithelium. Herein, we describe a protocol for CD4(+)CD8αα(+) T cell induction from freshly isolated naive CD4(+) T cells, including procedures for the isolation and enrichment of mou...

Descripción completa

Detalles Bibliográficos
Autores principales: Harada, Yosuke, Miyamoto, Kentaro, Sujino, Tomohisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526228/
https://www.ncbi.nlm.nih.gov/pubmed/36170110
http://dx.doi.org/10.1016/j.xpro.2022.101728
_version_ 1784800833732345856
author Harada, Yosuke
Miyamoto, Kentaro
Sujino, Tomohisa
author_facet Harada, Yosuke
Miyamoto, Kentaro
Sujino, Tomohisa
author_sort Harada, Yosuke
collection PubMed
description Recent studies have shown that CD4(+)CD8αα(+) T cells are induced in the hypoxic environment of the small intestinal epithelium. Herein, we describe a protocol for CD4(+)CD8αα(+) T cell induction from freshly isolated naive CD4(+) T cells, including procedures for the isolation and enrichment of mouse splenic T cells. In addition, we present an approach that can induce more CD4(+)CD8αα(+) T cells by artificially creating a hypoxic environment in vitro. For complete details on the use and execution of this protocol, please refer to Harada et al. (2022).
format Online
Article
Text
id pubmed-9526228
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-95262282022-10-02 Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction Harada, Yosuke Miyamoto, Kentaro Sujino, Tomohisa STAR Protoc Protocol Recent studies have shown that CD4(+)CD8αα(+) T cells are induced in the hypoxic environment of the small intestinal epithelium. Herein, we describe a protocol for CD4(+)CD8αα(+) T cell induction from freshly isolated naive CD4(+) T cells, including procedures for the isolation and enrichment of mouse splenic T cells. In addition, we present an approach that can induce more CD4(+)CD8αα(+) T cells by artificially creating a hypoxic environment in vitro. For complete details on the use and execution of this protocol, please refer to Harada et al. (2022). Elsevier 2022-09-27 /pmc/articles/PMC9526228/ /pubmed/36170110 http://dx.doi.org/10.1016/j.xpro.2022.101728 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Harada, Yosuke
Miyamoto, Kentaro
Sujino, Tomohisa
Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
title Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
title_full Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
title_fullStr Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
title_full_unstemmed Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
title_short Protocol to isolate and enrich mouse splenic naive CD4(+) T cells for in vitro CD4(+)CD8αα(+) cell induction
title_sort protocol to isolate and enrich mouse splenic naive cd4(+) t cells for in vitro cd4(+)cd8αα(+) cell induction
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526228/
https://www.ncbi.nlm.nih.gov/pubmed/36170110
http://dx.doi.org/10.1016/j.xpro.2022.101728
work_keys_str_mv AT haradayosuke protocoltoisolateandenrichmousesplenicnaivecd4tcellsforinvitrocd4cd8aacellinduction
AT miyamotokentaro protocoltoisolateandenrichmousesplenicnaivecd4tcellsforinvitrocd4cd8aacellinduction
AT sujinotomohisa protocoltoisolateandenrichmousesplenicnaivecd4tcellsforinvitrocd4cd8aacellinduction

Ejemplares similares