Protocol for preparing sensor molecules and analyzing heterotypic endomembrane fusion in insulin-responsive cells using live-cell imaging

Heterotypic endomembrane fusion between static GLUT4-containing vesicles and traveling transferrin receptor-containing endosomes triggers insulin-responsive translocation of the GLUT4 glucose transporter. Here, we provide a protocol for preparing BODIPY-based fluorescent sensor molecules allowing de...

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Detalles Bibliográficos
Autores principales: Hatakeyama, Hiroyasu, Kanzaki, Makoto
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526234/
https://www.ncbi.nlm.nih.gov/pubmed/36170109
http://dx.doi.org/10.1016/j.xpro.2022.101726

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526234/
https://www.ncbi.nlm.nih.gov/pubmed/36170109
http://dx.doi.org/10.1016/j.xpro.2022.101726

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