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Establishment and application of a CRISPR-Cas12a-based RPA-LFS and fluorescence for the detection of Trichomonas vaginalis

BACKGROUND: Infection with Trichomonas vaginalis can lead to cervicitis, urethritis, pelvic inflammatory disease, prostatitis and perinatal complications and increased risk of HIV transmission. Here, we used an RPA-based CRISPR-Cas12a assay system in combination with a lateral flow strip (LFS) (refe...

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Detalles Bibliográficos
Autores principales: Li, Shan, Wang, Xiaocen, Yu, Yanhui, Cao, Songgao, Liu, Juan, Zhao, Panpan, Li, Jianhua, Zhang, Xichen, Li, Xin, Zhang, Nan, Sun, Min, Cao, Lili, Gong, Pengtao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526244/
https://www.ncbi.nlm.nih.gov/pubmed/36180879
http://dx.doi.org/10.1186/s13071-022-05475-5