Higher off-target amplicon detection rate in MiSeq v3 compared to v2 reagent kits in the context of 16S-rRNA-sequencing

One of the most widely used techniques in microbiota research is 16S-rRNA-sequencing. Several laboratory processes have been shown to impact sequencing results, especially in low biomass samples. Low biomass samples are prone to off-target amplification, where instead of bacterial DNA, host DNA is e...

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Detalles Bibliográficos
Autores principales: Odendaal, Mari-Lee, Groot, James A., Hasrat, Raiza, Chu, Mei Ling J. N., Franz, Eelco, Bogaert, Debby, Bosch, Thijs, de Steenhuijsen Piters, Wouter A. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526709/
https://www.ncbi.nlm.nih.gov/pubmed/36183009
http://dx.doi.org/10.1038/s41598-022-20573-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9526709/
https://www.ncbi.nlm.nih.gov/pubmed/36183009
http://dx.doi.org/10.1038/s41598-022-20573-1

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