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Using pERK immunostaining to quantify neuronal activity induced by stress in zebrafish larvae

The larval zebrafish has emerged as a very useful model organism to study the neuronal circuits controlling neuroendocrine and behavioral responses to stress. This protocol describes how to expose zebrafish larvae to hyperosmotic stress and test whether candidate populations of neurons are activated...

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Detalles Bibliográficos
Autores principales: Corradi, Laura, Zaupa, Margherita, Sawamiphak, Suphansa, Filosa, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529592/
https://www.ncbi.nlm.nih.gov/pubmed/36183255
http://dx.doi.org/10.1016/j.xpro.2022.101731