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Mass spectrometry analysis of affinity-purified cytoplasmic translation initiation complexes from human and fly cells
eIF5-mimic protein (5MP) controls translation through binding to the ribosomal pre-initiation complex (PIC) and alters non-AUG translation rates for cancer oncogenes and repeat-expansions in neurodegenerative diseases. Here, we describe a semi-quantitative protocol for detecting 5MP-associated prote...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529597/ https://www.ncbi.nlm.nih.gov/pubmed/36181679 http://dx.doi.org/10.1016/j.xpro.2022.101739 |