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Mass spectrometry analysis of affinity-purified cytoplasmic translation initiation complexes from human and fly cells

eIF5-mimic protein (5MP) controls translation through binding to the ribosomal pre-initiation complex (PIC) and alters non-AUG translation rates for cancer oncogenes and repeat-expansions in neurodegenerative diseases. Here, we describe a semi-quantitative protocol for detecting 5MP-associated prote...

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Detalles Bibliográficos
Autores principales:	Singh, Chingakham Ranjit, Tani, Naoki, Nakamura, Akira, Asano, Katsura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529597/ https://www.ncbi.nlm.nih.gov/pubmed/36181679 http://dx.doi.org/10.1016/j.xpro.2022.101739

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