Direct isolation of single cells from living brains of Drosophila melanogaster without dissociation for transcriptome analysis

Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then outline the procedure...

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Detalles Bibliográficos
Autores principales: Barros, Claudia S., Bossing, Torsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529598/
https://www.ncbi.nlm.nih.gov/pubmed/36181682
http://dx.doi.org/10.1016/j.xpro.2022.101735

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529598/
https://www.ncbi.nlm.nih.gov/pubmed/36181682
http://dx.doi.org/10.1016/j.xpro.2022.101735

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