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Direct isolation of single cells from living brains of Drosophila melanogaster without dissociation for transcriptome analysis
Here, we describe a protocol to remove single identified cells directly from Drosophila living brains and analyze their transcriptome. We detail the steps to harvest fluorescent cells using a capillary under epifluorescence and transmitted light to avoid contamination. We then outline the procedure...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9529598/ https://www.ncbi.nlm.nih.gov/pubmed/36181682 http://dx.doi.org/10.1016/j.xpro.2022.101735 |