Native protein purification of ferroxidase LPR1 from leaf extracts of a transgenic Arabidopsis thaliana line

LPR1 (LOW PHOSPHATE ROOT 1), a bacterial-type plant ferroxidase, is crucial for local root phosphate (Pi) sensing. Here, we present a detailed protocol for native (tag-free) protein purification of LPR1 from leaf extracts by differential ammonium sulfate precipitation, size exclusion, and cation exc...

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Detalles Bibliográficos
Autores principales: Tang, Nancy, Naumann, Christin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530668/
https://www.ncbi.nlm.nih.gov/pubmed/36181680
http://dx.doi.org/10.1016/j.xpro.2022.101733

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530668/
https://www.ncbi.nlm.nih.gov/pubmed/36181680
http://dx.doi.org/10.1016/j.xpro.2022.101733

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