Cargando…
Native protein purification of ferroxidase LPR1 from leaf extracts of a transgenic Arabidopsis thaliana line
LPR1 (LOW PHOSPHATE ROOT 1), a bacterial-type plant ferroxidase, is crucial for local root phosphate (Pi) sensing. Here, we present a detailed protocol for native (tag-free) protein purification of LPR1 from leaf extracts by differential ammonium sulfate precipitation, size exclusion, and cation exc...
| Autores principales: | , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530668/ https://www.ncbi.nlm.nih.gov/pubmed/36181680 http://dx.doi.org/10.1016/j.xpro.2022.101733 |