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A step-by-step protocol for capturing conformational snapshots of ligand gated ion channels by single-particle cryo-EM
Capturing conformational snapshots by single-particle cryo-EM facilitates the analysis of ligand binding and activation mechanisms for ion channels and other receptor complexes. Here, we present a protocol to capture intermediate states of nanodisc-reconstituted TRPV1. This protocol covers sample pr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530669/ https://www.ncbi.nlm.nih.gov/pubmed/36181684 http://dx.doi.org/10.1016/j.xpro.2022.101732 |