A step-by-step protocol for capturing conformational snapshots of ligand gated ion channels by single-particle cryo-EM

Capturing conformational snapshots by single-particle cryo-EM facilitates the analysis of ligand binding and activation mechanisms for ion channels and other receptor complexes. Here, we present a protocol to capture intermediate states of nanodisc-reconstituted TRPV1. This protocol covers sample pr...

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Detalles Bibliográficos
Autores principales: Zhang, Kaihua, Julius, David, Cheng, Yifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530669/
https://www.ncbi.nlm.nih.gov/pubmed/36181684
http://dx.doi.org/10.1016/j.xpro.2022.101732

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