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Imaging and quantification of apical microvilli in the syncytial blastoderm of Drosophila embryos

The syncytial Drosophila blastoderm embryo contains apical microvilli with filamentous actin that are remodeled during nuclear division cycles 10–13. Here, we describe a protocol for preparing whole embryo samples and capturing images of microvilli using confocal and super-resolution STED microscopy...

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Detalles Bibliográficos
Autores principales: Mitra, Debasmita, Swaminathan, Amruta, Mundhe, Gayatri, Rikhy, Richa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530672/
https://www.ncbi.nlm.nih.gov/pubmed/36181681
http://dx.doi.org/10.1016/j.xpro.2022.101736