The use of CRISPR to generate a whole-gene humanized MAPT and the examination of P301L and G272V clinical variants, along with the creation of deletion null alleles of ptl-1, pgrn-1 and alfa-1 loci

To study important genes involved in Frontotemporal Dementia ( MAPT , GRN and C9orf72 ), we created deletion alleles in the three orthologous genes ( ptl-1 , pgrn-1 , and alfa-1 ). Simultaneously, we replaced the C. elegans ptl-1 gene with the predicted orthologous human MAPT gene, often called whol...

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Detalles Bibliográficos
Autores principales: Lins, Jeremy, Hopkins, Christopher E., Brock, Trisha, Hart, Anne C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530923/
https://www.ncbi.nlm.nih.gov/pubmed/36204657
http://dx.doi.org/10.17912/micropub.biology.000615

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9530923/
https://www.ncbi.nlm.nih.gov/pubmed/36204657
http://dx.doi.org/10.17912/micropub.biology.000615

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