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Detecting molecular interactions in live-cell single-molecule imaging with proximity-assisted photoactivation (PAPA)

Single-molecule imaging provides a powerful way to study biochemical processes in live cells, yet it remains challenging to track single molecules while simultaneously detecting their interactions. Here, we describe a novel property of rhodamine dyes, proximity-assisted photoactivation (PAPA), in wh...

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Detalles Bibliográficos
Autores principales: Graham, Thomas GW, Ferrie, John Joseph, Dailey, Gina M, Tjian, Robert, Darzacq, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9531946/
https://www.ncbi.nlm.nih.gov/pubmed/35976226
http://dx.doi.org/10.7554/eLife.76870