Dual-probe real-time PCR assay for detection of variola or other orthopoxviruses with dried reagents

A real-time, multiplexed polymerase chain reaction (PCR) assay based on dried PCR reagents was developed. Only variola virus could be specifically detected by a FAM (6-carboxyfluorescein)-labeled probe while camelpox, cowpox, monkeypox and vaccinia viruses could be detected by a TET (6-carboxytetram...

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Detalles Bibliográficos
Autores principales: Aitichou, Mohamed, Saleh, Sharron, Kyusung, Park, Huggins, John, O’Guinn, Monica, Jahrling, Peter, Ibrahim, Sofi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. Published by Elsevier B.V. 2008
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534013/
https://www.ncbi.nlm.nih.gov/pubmed/18725245
http://dx.doi.org/10.1016/j.jviromet.2008.07.018

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534013/
https://www.ncbi.nlm.nih.gov/pubmed/18725245
http://dx.doi.org/10.1016/j.jviromet.2008.07.018

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