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Dual-probe real-time PCR assay for detection of variola or other orthopoxviruses with dried reagents
A real-time, multiplexed polymerase chain reaction (PCR) assay based on dried PCR reagents was developed. Only variola virus could be specifically detected by a FAM (6-carboxyfluorescein)-labeled probe while camelpox, cowpox, monkeypox and vaccinia viruses could be detected by a TET (6-carboxytetram...
| Autores principales: | , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier B.V. Published by Elsevier B.V.
2008
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534013/ https://www.ncbi.nlm.nih.gov/pubmed/18725245 http://dx.doi.org/10.1016/j.jviromet.2008.07.018 |