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Association between rumen microbiota and marbling grade in Hu sheep
The marbling fat regulates the flavor of mutton and measures the fat density in the loin eye and is the most important parameter of carcass grading. The objective of this study was to explore the relationship of rumen microbiota and mutton marbling grade. One hundred and eighty-seven feedlot-finishe...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534374/ https://www.ncbi.nlm.nih.gov/pubmed/36212835 http://dx.doi.org/10.3389/fmicb.2022.978263 |
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author | Wang, Jianghui Zhang, Yukun Wang, Xiaojuan Li, Fadi Zhang, Deyin Li, Xiaolong Zhao, Yuan Zhao, Liming Xu, Dan Cheng, Jiangbo Li, Wenxin Lin, Changchun Yang, Xiaobin Zhai, Rui Zeng, Xiwen Cui, Panpan Ma, Zongwu Liu, Jia Zhang, Xiaoxue Wang, Weimin |
author_facet | Wang, Jianghui Zhang, Yukun Wang, Xiaojuan Li, Fadi Zhang, Deyin Li, Xiaolong Zhao, Yuan Zhao, Liming Xu, Dan Cheng, Jiangbo Li, Wenxin Lin, Changchun Yang, Xiaobin Zhai, Rui Zeng, Xiwen Cui, Panpan Ma, Zongwu Liu, Jia Zhang, Xiaoxue Wang, Weimin |
author_sort | Wang, Jianghui |
collection | PubMed |
description | The marbling fat regulates the flavor of mutton and measures the fat density in the loin eye and is the most important parameter of carcass grading. The objective of this study was to explore the relationship of rumen microbiota and mutton marbling grade. One hundred and eighty-seven feedlot-finished Hu male lambs (Age: 180 day; Final BW: 46.32 ± 6.03 kg) were slaughtered, and ruminal contents and marbling grade were collected. Ruminal microbial DNA extraction and 16S rRNA gene sequencing was performed to investigate microbial composition and to predict microbial metabolic pathways. The animal cohort was then grouped based on marbling grades [low marbling (LM), marbling grade ≤ 1; Medium marbling (MM), 1 < marbling grade ≤ 3; High Marbling (HM), 3 < marbling grade ≤ 5] and intramuscular fat-associated microorganisms were pinpointed using LEfSe and random forest classification model. Intramuscular fat content had significantly differences among the three groups (P < 0.05), and was significantly correlated with VFAs profiling. HM sheep showed a higher abundance of one bacterial taxon (Kandleria), and two taxa were overrepresented in the MM sheep (Pseudobutyrivibrio and Monoglobus), respectively. In addition, the main intramuscular fat deposition pathway was found to involve peroxisome proliferator-activated receptor (PPAR) fatty acid synthesis. By studying the effect of the ruminal microbiome on the marbling of sheep, the present study provides insights into the production of high-quality mutton. |
format | Online Article Text |
id | pubmed-9534374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95343742022-10-06 Association between rumen microbiota and marbling grade in Hu sheep Wang, Jianghui Zhang, Yukun Wang, Xiaojuan Li, Fadi Zhang, Deyin Li, Xiaolong Zhao, Yuan Zhao, Liming Xu, Dan Cheng, Jiangbo Li, Wenxin Lin, Changchun Yang, Xiaobin Zhai, Rui Zeng, Xiwen Cui, Panpan Ma, Zongwu Liu, Jia Zhang, Xiaoxue Wang, Weimin Front Microbiol Microbiology The marbling fat regulates the flavor of mutton and measures the fat density in the loin eye and is the most important parameter of carcass grading. The objective of this study was to explore the relationship of rumen microbiota and mutton marbling grade. One hundred and eighty-seven feedlot-finished Hu male lambs (Age: 180 day; Final BW: 46.32 ± 6.03 kg) were slaughtered, and ruminal contents and marbling grade were collected. Ruminal microbial DNA extraction and 16S rRNA gene sequencing was performed to investigate microbial composition and to predict microbial metabolic pathways. The animal cohort was then grouped based on marbling grades [low marbling (LM), marbling grade ≤ 1; Medium marbling (MM), 1 < marbling grade ≤ 3; High Marbling (HM), 3 < marbling grade ≤ 5] and intramuscular fat-associated microorganisms were pinpointed using LEfSe and random forest classification model. Intramuscular fat content had significantly differences among the three groups (P < 0.05), and was significantly correlated with VFAs profiling. HM sheep showed a higher abundance of one bacterial taxon (Kandleria), and two taxa were overrepresented in the MM sheep (Pseudobutyrivibrio and Monoglobus), respectively. In addition, the main intramuscular fat deposition pathway was found to involve peroxisome proliferator-activated receptor (PPAR) fatty acid synthesis. By studying the effect of the ruminal microbiome on the marbling of sheep, the present study provides insights into the production of high-quality mutton. Frontiers Media S.A. 2022-09-21 /pmc/articles/PMC9534374/ /pubmed/36212835 http://dx.doi.org/10.3389/fmicb.2022.978263 Text en Copyright © 2022 Wang, Zhang, Wang, Li, Zhang, Li, Zhao, Zhao, Xu, Cheng, Li, Lin, Yang, Zhai, Zeng, Cui, Ma, Liu, Zhang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Wang, Jianghui Zhang, Yukun Wang, Xiaojuan Li, Fadi Zhang, Deyin Li, Xiaolong Zhao, Yuan Zhao, Liming Xu, Dan Cheng, Jiangbo Li, Wenxin Lin, Changchun Yang, Xiaobin Zhai, Rui Zeng, Xiwen Cui, Panpan Ma, Zongwu Liu, Jia Zhang, Xiaoxue Wang, Weimin Association between rumen microbiota and marbling grade in Hu sheep |
title | Association between rumen microbiota and marbling grade in Hu sheep |
title_full | Association between rumen microbiota and marbling grade in Hu sheep |
title_fullStr | Association between rumen microbiota and marbling grade in Hu sheep |
title_full_unstemmed | Association between rumen microbiota and marbling grade in Hu sheep |
title_short | Association between rumen microbiota and marbling grade in Hu sheep |
title_sort | association between rumen microbiota and marbling grade in hu sheep |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534374/ https://www.ncbi.nlm.nih.gov/pubmed/36212835 http://dx.doi.org/10.3389/fmicb.2022.978263 |
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