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Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers

Cowpea [Vigna unguiculata (L.) Walp.] is a crop with significant agronomic and nutritional value. In Togo, the crop is very appreciated by local people. It is the third food habit in Togo after maize and rice. However, several accessions of cowpea cultivated in Togo are now prone to extinction, crea...

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Autores principales: Dagnon, Yao Dodzi, Palanga, Koffi Kibalou, Bammite, Damigou, Bodian, Amy, Akabassi, Ghislain Comlan, Foncéka, Daniel, Tozo, Koffi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534403/
https://www.ncbi.nlm.nih.gov/pubmed/36197899
http://dx.doi.org/10.1371/journal.pone.0252362
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author Dagnon, Yao Dodzi
Palanga, Koffi Kibalou
Bammite, Damigou
Bodian, Amy
Akabassi, Ghislain Comlan
Foncéka, Daniel
Tozo, Koffi
author_facet Dagnon, Yao Dodzi
Palanga, Koffi Kibalou
Bammite, Damigou
Bodian, Amy
Akabassi, Ghislain Comlan
Foncéka, Daniel
Tozo, Koffi
author_sort Dagnon, Yao Dodzi
collection PubMed
description Cowpea [Vigna unguiculata (L.) Walp.] is a crop with significant agronomic and nutritional value. In Togo, the crop is very appreciated by local people. It is the third food habit in Togo after maize and rice. However, several accessions of cowpea cultivated in Togo are now prone to extinction, creating a risk of genetic erosion. It is therefore urgent to assess the genetic diversity of accessions in order to set up a good conservation program. To achieve this, genetic diversity and phylogenetic relationships among 70 accessions of cowpea collected in the five (5) administrative regions of Togo were assessed using Simple Sequence Repeat (SSR) molecular markers. The twenty-eight SSR primers used in this study generated a total of 164 alleles with an average of 5.82 alleles per locus. Polymorphic Information Content (PIC) values ranged from 0.20 to 0.89 with an average value of 0.58. Population structure analysis using model-based revealed that the cowpea germplasm was grouped into two subpopulations. The analysis of molecular variance (AMOVA) revealed that 98% of genetic variation existed among accessions within regions. The fixation index (Fst) value, which was 0.069 was low, indicating relatively low population differentiation. The phylogenetic analysis grouped the 70 accessions into two main groups that can be further divided into four groups independent of their origins. This study provides a foundation for a Togolese cowpea germplasm conservation program and can serve for the selection of parental material for further studies aimed at the genetic improvement of local germplasm.
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spelling pubmed-95344032022-10-06 Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers Dagnon, Yao Dodzi Palanga, Koffi Kibalou Bammite, Damigou Bodian, Amy Akabassi, Ghislain Comlan Foncéka, Daniel Tozo, Koffi PLoS One Research Article Cowpea [Vigna unguiculata (L.) Walp.] is a crop with significant agronomic and nutritional value. In Togo, the crop is very appreciated by local people. It is the third food habit in Togo after maize and rice. However, several accessions of cowpea cultivated in Togo are now prone to extinction, creating a risk of genetic erosion. It is therefore urgent to assess the genetic diversity of accessions in order to set up a good conservation program. To achieve this, genetic diversity and phylogenetic relationships among 70 accessions of cowpea collected in the five (5) administrative regions of Togo were assessed using Simple Sequence Repeat (SSR) molecular markers. The twenty-eight SSR primers used in this study generated a total of 164 alleles with an average of 5.82 alleles per locus. Polymorphic Information Content (PIC) values ranged from 0.20 to 0.89 with an average value of 0.58. Population structure analysis using model-based revealed that the cowpea germplasm was grouped into two subpopulations. The analysis of molecular variance (AMOVA) revealed that 98% of genetic variation existed among accessions within regions. The fixation index (Fst) value, which was 0.069 was low, indicating relatively low population differentiation. The phylogenetic analysis grouped the 70 accessions into two main groups that can be further divided into four groups independent of their origins. This study provides a foundation for a Togolese cowpea germplasm conservation program and can serve for the selection of parental material for further studies aimed at the genetic improvement of local germplasm. Public Library of Science 2022-10-05 /pmc/articles/PMC9534403/ /pubmed/36197899 http://dx.doi.org/10.1371/journal.pone.0252362 Text en © 2022 Dagnon et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dagnon, Yao Dodzi
Palanga, Koffi Kibalou
Bammite, Damigou
Bodian, Amy
Akabassi, Ghislain Comlan
Foncéka, Daniel
Tozo, Koffi
Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers
title Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers
title_full Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers
title_fullStr Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers
title_full_unstemmed Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers
title_short Genetic diversity and population structure of cowpea [Vigna unguiculata (L.) Walp.] accessions from Togo using SSR markers
title_sort genetic diversity and population structure of cowpea [vigna unguiculata (l.) walp.] accessions from togo using ssr markers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9534403/
https://www.ncbi.nlm.nih.gov/pubmed/36197899
http://dx.doi.org/10.1371/journal.pone.0252362
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