A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells

Microglia are implicated in most neurodegenerative diseases. Here, we present a robust and efficient protocol to differentiate monocyte-derived microglia-like cells (MDMi) from whole blood. The protocol consists of three parts. The first part will describe two methods for PBMC isolation. This will b...

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Detalles Bibliográficos
Autores principales: Quek, Hazel, Cuní-López, Carla, Stewart, Romal, Lim, Yi Chieh, Roberts, Tara L., White, Anthony R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535318/
https://www.ncbi.nlm.nih.gov/pubmed/36201317
http://dx.doi.org/10.1016/j.xpro.2022.101747

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535318/
https://www.ncbi.nlm.nih.gov/pubmed/36201317
http://dx.doi.org/10.1016/j.xpro.2022.101747

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