A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells

Microglia are implicated in most neurodegenerative diseases. Here, we present a robust and efficient protocol to differentiate monocyte-derived microglia-like cells (MDMi) from whole blood. The protocol consists of three parts. The first part will describe two methods for PBMC isolation. This will b...

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Detalles Bibliográficos
Autores principales: Quek, Hazel, Cuní-López, Carla, Stewart, Romal, Lim, Yi Chieh, Roberts, Tara L., White, Anthony R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535318/
https://www.ncbi.nlm.nih.gov/pubmed/36201317
http://dx.doi.org/10.1016/j.xpro.2022.101747
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author Quek, Hazel
Cuní-López, Carla
Stewart, Romal
Lim, Yi Chieh
Roberts, Tara L.
White, Anthony R.
author_facet Quek, Hazel
Cuní-López, Carla
Stewart, Romal
Lim, Yi Chieh
Roberts, Tara L.
White, Anthony R.
author_sort Quek, Hazel
collection PubMed
description Microglia are implicated in most neurodegenerative diseases. Here, we present a robust and efficient protocol to differentiate monocyte-derived microglia-like cells (MDMi) from whole blood. The protocol consists of three parts. The first part will describe two methods for PBMC isolation. This will be followed by MDMi differentiation, and lastly, the characterization of MDMi by immunocytochemistry. MDMi can be used to investigate microglial-related responses in various age-related neurodegenerative diseases and can be applied to drug testing on a personalized basis. For complete details on the use and execution of this protocol, please refer to Quek et al. (2022).
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spelling pubmed-95353182022-10-07 A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells Quek, Hazel Cuní-López, Carla Stewart, Romal Lim, Yi Chieh Roberts, Tara L. White, Anthony R. STAR Protoc Protocol Microglia are implicated in most neurodegenerative diseases. Here, we present a robust and efficient protocol to differentiate monocyte-derived microglia-like cells (MDMi) from whole blood. The protocol consists of three parts. The first part will describe two methods for PBMC isolation. This will be followed by MDMi differentiation, and lastly, the characterization of MDMi by immunocytochemistry. MDMi can be used to investigate microglial-related responses in various age-related neurodegenerative diseases and can be applied to drug testing on a personalized basis. For complete details on the use and execution of this protocol, please refer to Quek et al. (2022). Elsevier 2022-10-05 /pmc/articles/PMC9535318/ /pubmed/36201317 http://dx.doi.org/10.1016/j.xpro.2022.101747 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Quek, Hazel
Cuní-López, Carla
Stewart, Romal
Lim, Yi Chieh
Roberts, Tara L.
White, Anthony R.
A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
title A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
title_full A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
title_fullStr A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
title_full_unstemmed A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
title_short A robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
title_sort robust approach to differentiate human monocyte-derived microglia from peripheral blood mononuclear cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535318/
https://www.ncbi.nlm.nih.gov/pubmed/36201317
http://dx.doi.org/10.1016/j.xpro.2022.101747
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