Expanded targeting scope of LbCas12a variants allows editing of multiple oncogenic mutations

RNA-guided CRISPR-Cas12a endonucleases are promising tools for genome engineering. Here we demonstrate that LbCas12a variants derived from Lachnospiraceae bacterium show a broad PAM preference, recognizing certain non-canonical PAMs with high efficiency. Furthermore, we engineered LbABE8e to carry G...

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Detalles Bibliográficos
Autores principales: Choi, Eunyoung, Hwang, Hye-Yeon, Kwon, Eunji, Kim, Daesik, Koo, Taeyoung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535386/
https://www.ncbi.nlm.nih.gov/pubmed/36250202
http://dx.doi.org/10.1016/j.omtn.2022.09.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535386/
https://www.ncbi.nlm.nih.gov/pubmed/36250202
http://dx.doi.org/10.1016/j.omtn.2022.09.005

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