Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells

The PARP-1 expression level and poly (ADP-ribosyl)ation activity in cancer markedly affect the therapeutic outcome. Nicaraven, a free radical scavenger has been found to inhibit PARP, but the effect on cancer cells is still unclear. In this study, we investigated the potential role and molecular mec...

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Autores principales: Abdelghany, Lina, Kawabata, Tsuyoshi, Goto, Shinji, Jingu, Keiichi, Li, Tao-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Neoplasia Press 2022
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535466/
https://www.ncbi.nlm.nih.gov/pubmed/36206675
http://dx.doi.org/10.1016/j.tranon.2022.101548
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author Abdelghany, Lina
Kawabata, Tsuyoshi
Goto, Shinji
Jingu, Keiichi
Li, Tao-Sheng
author_facet Abdelghany, Lina
Kawabata, Tsuyoshi
Goto, Shinji
Jingu, Keiichi
Li, Tao-Sheng
author_sort Abdelghany, Lina
collection PubMed
description The PARP-1 expression level and poly (ADP-ribosyl)ation activity in cancer markedly affect the therapeutic outcome. Nicaraven, a free radical scavenger has been found to inhibit PARP, but the effect on cancer cells is still unclear. In this study, we investigated the potential role and molecular mechanism of nicaraven on cancer cells. Using U937 lymphoma cells and HCT-8 colorectal cancer cells, we found that nicaraven moderately reduced the cell viability of both cells in a dose-dependent manner. Interestingly, nicaraven significantly induced apoptosis of U937 cells that are dominantly expressing Bcl-2 but induced PAR-dependent cell death (parthanatos) of HCT-8 cells that are highly expressing poly (ADP-ribose) glycohydrolase (PARG). Based on our data, nicaraven seems to induce programmed cell death through distinct mechanisms, according to the expression levels of Bcl-2 and PARG in cancer cells.
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spelling pubmed-95354662022-10-14 Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells Abdelghany, Lina Kawabata, Tsuyoshi Goto, Shinji Jingu, Keiichi Li, Tao-Sheng Transl Oncol Original Research The PARP-1 expression level and poly (ADP-ribosyl)ation activity in cancer markedly affect the therapeutic outcome. Nicaraven, a free radical scavenger has been found to inhibit PARP, but the effect on cancer cells is still unclear. In this study, we investigated the potential role and molecular mechanism of nicaraven on cancer cells. Using U937 lymphoma cells and HCT-8 colorectal cancer cells, we found that nicaraven moderately reduced the cell viability of both cells in a dose-dependent manner. Interestingly, nicaraven significantly induced apoptosis of U937 cells that are dominantly expressing Bcl-2 but induced PAR-dependent cell death (parthanatos) of HCT-8 cells that are highly expressing poly (ADP-ribose) glycohydrolase (PARG). Based on our data, nicaraven seems to induce programmed cell death through distinct mechanisms, according to the expression levels of Bcl-2 and PARG in cancer cells. Neoplasia Press 2022-10-04 /pmc/articles/PMC9535466/ /pubmed/36206675 http://dx.doi.org/10.1016/j.tranon.2022.101548 Text en © 2022 The Authors. Published by Elsevier Inc. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Research
Abdelghany, Lina
Kawabata, Tsuyoshi
Goto, Shinji
Jingu, Keiichi
Li, Tao-Sheng
Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
title Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
title_full Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
title_fullStr Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
title_full_unstemmed Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
title_short Nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of Bcl-2 and poly (ADP-ribose) glycohydrolase in cancer cells
title_sort nicaraven induces programmed cell death by distinct mechanisms according to the expression levels of bcl-2 and poly (adp-ribose) glycohydrolase in cancer cells
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535466/
https://www.ncbi.nlm.nih.gov/pubmed/36206675
http://dx.doi.org/10.1016/j.tranon.2022.101548
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